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Non-Field Guidance and Technical Assistance

Municipal, Industrial, Commercial and Other Laboratories

The information contained on these pages is meant to provide technical assistance and guidance for meeting the requirements of NC WW/GW Laboratory Certification. On this page you will find guidance documents and technical assistance as well as Approved Procedures for Field Parameters. The following pages contain policies and checklists pertaining to laboratory operations.

Guidance and Technical Assistance Materials

Guidance documents are meant to provide detailed information for meeting policy or regulatory requirements.

Document Description
1631 LL Hg Preservation Clarification Letter from EPA Region 4 clarifying the preservation and hold time requirements found in EPA 1631, Rev. E and 40 CFR 136.3 Table II Footnote 17.
 MDL Presentation  Lab Tech Day 2018 presentation about the new MDL procedure.
Standard Methods QC Requirements Chart Spreadsheet outlining QC requirements for Standard Methods methods per the QC chapters (e.g., 2020, 3020, 4020, etc.)
Fecal Coliform (MF) Colony Confirmation Video This video provides a visual example of most elements involved in performing the monthly fecal coliform colony verification required for laboratories certified for Standard Methods 9222D. Please use this in conjunction with the technical assistance document linked below to assure all requirements are met and to further explain the process.
Fecal MF Colony Verification Technical Assistance Assistance in performing the required monthly colony verification for Fecal Coliform (Membrane Filter)
Fecal MF Colony Verification Example Benchsheet Example benchsheet to use when performing the colony verification and adjusting colony counts
Procedure for Testing Consumable Materials for Biological Testing A procedure for verifying the efficacy of new lots of consumable materials used for biological testing 
Fecal Coliform Reporting Guidelines Guidelines for enumerating and reporting fecal coliform results
MPN Table with 95% Confidence Ranges Table provided by IDEXX with MPN results and 95% confidence ranges using the Quanti-Tray/2000 which can be used to evaluate duplicate precision. 
LDO Daily Calibration Verification Procedure A procedure for verifying the calibration of Luminescence Dissolved Oxygen (LDO) meters by back calculation with solubility table
Calibration of Pipettors An example procedure for the gravimetric verification of mechanical liquid dispensing devices 
Matrix Spiking Technical Assistance Document A document describing appropriate matrix spiking frequencies and practices 
Memo to Permittees Regarding TRC 50 µg/L Compliance Level  A memo from the NC DWQ Point Source Branch describing the 50 ppb compliance level for Total Residual Chlorine (TRC)
Corrective Action Response to Audit Findings Guidance A document describing the corrective action response and documentation needed for labs to address Findings from audits. 
Ammonia/Fluoride Distillation Technical Assistance Document 

A document describing the background and implementation of Ammonia and Fluoride distillation study requirements in accordance with 40 CFR Part 136.3, Table IB, footnote 6.

Ammonia/Fluoride Distillation Flow Chart A flow chart showing how to set up the distillation study (see Tools table below for a calculation spreadsheet)

Tools

This table provides computer applications, spreadsheets, calculators, etc. to aid laboratories and laboratory analysts in meeting certification and quality assurance requirements.

Tool Description
 MDL Calculator  Spreadsheet used to calculate initial and/or on-going MDL.
Ammonia/Fluoride Distillation calculation spreadsheet A spreadsheet that can be used to evaluate the distillation study results

Total Residual Chorine (TRC) Curve Calculators

Excel® spreadsheets for calculating the correlation coefficient and percent recoveries of standards used to verify the instrument calibration. The worksheets may also be used to document the process and satisfy record retention requirements.
Free Available Chlorine (FAC) Curve Calculator An Excel® spreadsheet for calculating the correlation coefficient and percent recoveries of standards used to verify the instrument calibration. The worksheet may also be used to document the process and satisfy record retention requirements.
Adjustable Pipettor Calibration Worksheet An Excel® spreadsheet for determining relative error and coefficient of variation of adjustable pipettors. An example procedure for calibrating mechanical volumetric liquid-dispensing devices can be viewed here.
Fixed Pipettor Calibration Worksheet  An Excel® spreadsheet for determining relative error and coefficient of variation of fixed pipettors. An example procedure for calibrating mechanical volumetric liquid-dispensing devices can be viewed here.
ADMI Color Calculation Spreadsheet  An Excel® spreadsheet for calculating ADMI color using the Tristimulus Filter Method. Tristimulus values are converted to an ADMI single number color difference of all the same magnitude assigned to PtCo standards using the Adams Nickerson Color Difference (DE). 
Total Hardness (mg/L as CaCO3) Calculator An Excel® spreadsheet for calculating Total Hardness (mg/L as CaCO3) using results obtained for Calcium and Magnesium.
SOUR Calculation and Temperature Correction for pH Link to Land Application Unit's VAR Option 4 spreadsheets

Approved Procedures for the Analysis of Field Parameters

These documents are intended to provide the approved procedures and requirements for measurement of Field parameters. You may refer to the approved method reference, instrument manufacturer's manual or other resources for additional information. They can be downloaded from the Field lab procedures and technical assistance page of this website.

 

Link to National Pollution Discharge Elimination System (NPDES) Permitting Guidance, Directions for Completing a Discharge Monitoring Report (DMR) and Calculating Data for DMR reporting. Look under Compliance Forms. You can also find a link to the list of Significant Figures and Their Use on DMRs document and DMR parameter code descriptions on that page. [NOTE: The parameter code for any Fecal coliform regardless of technology (e.g., Colilert-18, MF, MPN/100 mL, etc.) code is now 31616]

 

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